Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.
Berger, I., Garzoni, F., Chaillet, M., Haffke, M., Gupta, K., Aubert, A. The MultiBac Protein Complex Production Platform at the EMBL. J. Vis. Exp. (77), e50159, doi:10.3791/50159 (2013).