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Chapters
- 00:05Title
- 02:13Tandem Recombineering (TR) for Creating Multigene Expression Constructs
- 04:57Composite Multigene Baculovirus Generation, Amplification and Storage
- 05:02Baculovirus Generation
- 06:42Amplification
- 08:14Storage
- 08:55Protein Production and Downstream Processing
- 08:57Infecting Larger Cultures and Monitoring YFP
- 10:20Cell Lysis and Fractionation
- 10:45Protein Purification
- 11:07Future Outlook
- 11:33Results: Production of Eukaryotic Protein Complexes by MultiBac
- 12:18Conclusion
Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.
