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Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research
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Chapters
- 00:07Introduction
- 01:17CTC Amplification in 3D Culture Conditions
- 02:01Immuno-Fluorescence Staining on CTC Sphere Sections
- 02:41Results: Immuno-Fluorescence Staining on CTC Sphere Sections
- 02:52Cytometry Analysis
- 03:50Results: Cytometry Analysis
- 04:13Luminescence Visability Assay
- 05:05Results: Luminescence Visability Assay
- 05:14Subcutaneous Injections
- 05:32Results: Subcutaneous Injections
- 05:42Intrasplenic Injection
- 06:27Results: Intrasplenic Injection
- 06:42Conclusion
Culturing CTCs allows a deeper functional characterization of cancer, through assaying specific marker expression, and assessing drug resistance and the ability to colonize the liver among other possibilities. Overall, CTC culture could be a promising clinical tool for personalized medicine to improve patient outcome.
