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Journal / Cancer Research / 使用膜结合IL-21修饰B细胞系的自然杀伤（NK）和CAR-NK细胞扩增方法…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
使用膜结合IL-21修饰B细胞系的自然杀伤（NK）和CAR-NK细胞扩增方法
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使用膜结合IL-21修饰B细胞系的自然杀伤（NK）和CAR-NK细胞扩增方法

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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在这里，我们提出了一种扩增外周血自然杀伤（PBNK），来自肝组织的NK细胞以及源自外周血单核细胞（PBMC）或脐带血（CB）的嵌合抗原受体（CAR）-NK细胞的方法。该协议展示了使用221-mIL-21饲养层细胞的NK和CAR-NK细胞的扩增，以及扩增的NK细胞的优化纯度。

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癌症研究，第180期，
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