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Cancer Research
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磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现
JoVE Journal
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JoVE Journal
Cancer Research
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
Please note that all translations are automatically generated.
Click here for the English version.
磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现
DOI:
10.3791/58386-v
•
08:38 min
•
October 04, 2018
•
Sigrid S. Skånland
2
1
Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership
,
University of Oslo
,
2
K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy
,
University of Oslo
Chapters
00:04
Title
00:32
Preparation, Stimulation, and Fixation of Cells
01:50
Fluorescent Cell Barcoding
03:30
Cell Permeabilization and Intracellular Antigen Staining
06:12
Results: Phospho Flow Cytometry Analysis with Fluorescent Cell Barcoding
08:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 提出了一种中-高通量分析在细胞级蛋白质磷酸化事件的协议。磷流式细胞术是表征信号畸变、识别和验证生物标志物、评估药效学的有力手段。
Tags
Phospho Flow Cytometry
Fluorescent Cell Barcoding
Single Cell Signaling Analysis
Biomarker Discovery
Cell Biology
Immunology
Signaling Pathways
Stimuli
Drugs
High Throughput
RPMI 16/40 Medium
Fixed Buffer
Anti-IgM
PBS
Barcoding Reagents
Perm Buffer
Compensation Control
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