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Journal / Bioengineering / Generering af en forenklet tredimensionel…
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Bioengineering

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JoVE Journal Bioengineering
Generering af en forenklet tredimensionel skin-on-a-chip-model i en mikromaskiner med mikrofluidisk platform
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Generering af en forenklet tredimensionel skin-on-a-chip-model i en mikromaskiner med mikrofluidisk platform

Article DOI: 10.3791/62353-v • 06:30 min • May 17th, 2021
May 17th, 2021 •
1, 1, 2, 3, 1,4
1Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology, Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine, CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón

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Her præsenterer vi en protokol til at generere en tredimensionel forenklet og udifferentieret hudmodel ved hjælp af en mikromaskineret mikrofluidisk platform. En parallel flow tilgang gør det muligt in situ deposition af et dermalt rum til såning af epitelceller på toppen, alle styres af sprøjte pumper.

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