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Generering af en forenklet tredimensionel skin-on-a-chip-model i en mikromaskiner med mikrofluidisk platform
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1Department of Bioengineering and Aerospace Engineering,Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology,Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine,CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón
Chapters
- 00:04Introduction
- 00:48Chip Design and Micromachining Parameters
- 01:44Chip Assembly
- 02:43Pump Connections and Parallel Flow Protocol
- 03:55Human Immortalized Skin Keratinocytes (hKCs) Monolayer Seeding
- 04:31Results: Characterization of Skin Model in The Microfluidic Chip with Confocal and Fluorescence Microscopy
- 05:49Conclusion
Her præsenterer vi en protokol til at generere en tredimensionel forenklet og udifferentieret hudmodel ved hjælp af en mikromaskineret mikrofluidisk platform. En parallel flow tilgang gør det muligt in situ deposition af et dermalt rum til såning af epitelceller på toppen, alle styres af sprøjte pumper.
