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Journal
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Cancer Research
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ゼブラフィッシュラーバル異種移植片の生成と腫瘍行動解析
JoVE Journal
Cancer Research
Author Produced
This content is Free Access.
JoVE Journal
Cancer Research
Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
Please note that all translations are automatically generated.
Click here for the English version.
ゼブラフィッシュラーバル異種移植片の生成と腫瘍行動解析
DOI:
10.3791/62373-v
•
12:08 min
•
June 19, 2021
•
Mayra Martinez-Lopez
2
,
Vanda Póvoa
,
Rita Fior
1
Champalimaud Centre for the Unknown, Champalimaud Foundation
,
2
Instituto Gulbenkian de Ciência
Chapters
00:00
Introduction
01:37
Setting Up for Injection
02:04
24 Hours Before Injection
02:48
Injection Day
04:24
Injection Procedures
06:12
Metastasic Assay
07:10
4 Day Post-Injection
08:05
Whole Mount Immunostining for Confocal Imaging
08:35
Mounting of Xenografts
09:02
Confocal Imaging
10:22
Representative Results
11:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、異種移植片を生成するためのヒントと腫瘍行動分析、全実装免疫蛍光、共焦点イメージング定量のためのガイドラインを含むステップバイステップのプロトコルを提供します。
Tags
Zebrafish Xenografts
Cancer Research
Larva Model
Proliferation
Angiogenesis
Metastasis
Tumor Microenvironment
Anti-cancer Therapies
Patient-derived Cells
Cell Injection
In-vitro Confluence
Zebrafish Embryos
Agarose Plates
Microinjection
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