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Biology
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外分泌物的自动检测和分析
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Biology
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JoVE Journal
Biology
Automated Detection and Analysis of Exocytosis
Please note that all translations are automatically generated.
Click here for the English version.
外分泌物的自动检测和分析
DOI:
10.3791/62400-v
•
13:28 min
•
September 11, 2021
•
Fabio Urbina
,
Stephanie L. Gupton
1
UNC Neuroscience Center, Department of Cell Biology and Physiology
,
University of North Carolina at Chapel Hill
Chapters
00:00
Introduction
00:44
Protocol
11:26
Representative Results
12:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们开发了自动计算机视觉软件，以检测以pH敏感荧光探头为标志的外细胞事件。在这里，我们演示了使用图形用户界面和 RStudio 来检测聚变事件、分析和显示聚变空间参数，并将事件分类为不同的聚变模式。
Tags
Keywords: Exocytosis
Automated Detection
Analysis
TIRF Imaging
PH-sensitive Fluorophores
Exocytic Events
Spatial Distribution
Frequency
Half-life
Fluorescence Change
Exocytosis Modes
Data Files
Mask Files
Frame Rate
Pixel Size
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