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Journal / Biochemistry / Proteomisk profil av EPS-urin gjennom FASP…
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Biochemistry
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JoVE Journal Biochemistry
Proteomisk profil av EPS-urin gjennom FASP fordøyelse og datauavhengig analyse
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Proteomisk profil av EPS-urin gjennom FASP fordøyelse og datauavhengig analyse

Article DOI: 10.3791/62512-v • 14:48 min • May 8th, 2021
May 8th, 2021 •
1, 1, 2, 3, 3, 1, 4, 1
1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 2Department of Health Science, Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro

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Her presenterer vi en optimalisert fordøyelsesprotokoll på filteret med detaljert informasjon om følgende: protein fordøyelse, peptidrensing og datauavhengig anskaffelsesanalyse. Denne strategien brukes til analyse av uttrykte prostatiske sekreter-urinprøver og tillater høy proteomdekning og lav manglende verdi etikettfri profilering av urinproteomet.

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