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Profilazione della modifica H3K4me3 negli impianti utilizzando la scissione sotto target e tagmentazione
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Profilazione della modifica H3K4me3 negli impianti utilizzando la scissione sotto target e tagmentazione

Article DOI: 10.3791/62534-v • 09:48 min • April 22nd, 2022
April 22nd, 2022 •
*1,2, *3, *1, 1,4
1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University
* These authors contributed equally

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La scissione sotto bersagli e tagmentazione (CUT&Tag) è un'efficiente strategia di profilazione epigenomica della cromatina. Questo protocollo presenta una raffinata strategia CUT&Tag per la profilazione delle modificazioni istoniche negli impianti.

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Biologia Numero 182
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