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Profilierung der H3K4me3-Modifikation in Pflanzen mittels Spaltung unter Targets und Tagmentation
JoVE Journal
Biology
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JoVE Journal Biology
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
DOI:

09:48 min

April 22, 2022

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Chapters

  • 00:06Introduction
  • 01:51Nuclei Isolation
  • 04:22Antibody Incubation
  • 06:15Transposase Incubation
  • 07:24Tagmentation
  • 07:52DNA Extraction and NGS Library Construction
  • 08:19Representative Results
  • 09:25Conclusion

Summary

Automatic Translation

Cleavage under targets and tagmentation (CUT&Tag) ist eine effiziente Chromatin-Epigenom-Profiling-Strategie. Dieses Protokoll stellt eine verfeinerte CUT&Tag-Strategie für die Profilierung von Histonmodifikationen in Pflanzen dar.

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