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Biology
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在靶标和标记下使用裂解分析植物中的H3K4me3修饰
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Biology
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JoVE Journal
Biology
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
Please note that all translations are automatically generated.
Click here for the English version.
在靶标和标记下使用裂解分析植物中的H3K4me3修饰
DOI:
10.3791/62534-v
•
09:48 min
•
April 22, 2022
•
Xiaoyuan Tao*
1,2
,
Mengtao Gao*
3
,
Siyuan Wang*
1
,
Xueying Guan
4
1
College of Agriculture and Biotechnology
,
Zhejiang University
,
2
Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
,
Zhejiang Academy of Agricultural Sciences
,
3
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture
,
Nanjing Agricultural University
,
4
Hainan Institute of Zhejiang University
Chapters
00:06
Introduction
01:51
Nuclei Isolation
04:22
Antibody Incubation
06:15
Transposase Incubation
07:24
Tagmentation
07:52
DNA Extraction and NGS Library Construction
08:19
Representative Results
09:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
靶标下裂解(CUT&Tag)是一种有效的染色质表观基因组分析策略。该协议提出了一种改进的CUT&Tag策略,用于分析植物中的组蛋白修饰。
Tags
H3K4me3 Modification
CUT&Tag
Chromatin Profiling
Histone Modification
Epigenomics
Cotton Leaves
Nuclear Isolation
Transposase
DNA Sequencing
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