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在靶标和标记下使用裂解分析植物中的H3K4me3修饰
JoVE Journal
Biology
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JoVE Journal Biology
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
DOI:

09:48 min

April 22, 2022

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Chapters

  • 00:06Introduction
  • 01:51Nuclei Isolation
  • 04:22Antibody Incubation
  • 06:15Transposase Incubation
  • 07:24Tagmentation
  • 07:52DNA Extraction and NGS Library Construction
  • 08:19Representative Results
  • 09:25Conclusion

Summary

Automatic Translation

靶标下裂解(CUT&Tag)是一种有效的染色质表观基因组分析策略。该协议提出了一种改进的CUT&Tag策略,用于分析植物中的组蛋白修饰。

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