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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
DOI:

09:19 min

June 01, 2022

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Chapters

  • 00:04Introduction
  • 00:59Stable Isotope Probing in Biological Samples Preparation
  • 02:17Sample Extraction
  • 04:10Analyze Samples on the Gas Chromatography Mass Spectrometer (GC-MS)
  • 04:56Data Analysis
  • 05:45Results: GC-MS, Heatmaps, and Data Analysis of the 13C-Labeled Volatiles in Fecal, Saliva, Sewage, Cultured and Uncultured Sputum Samples
  • 08:24Conclusion

Summary

Automatic Translation

This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing.

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