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Biochemistry

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JoVE Journal Biochemistry
Indfangning af aktivt producerede mikrobielle flygtige organiske forbindelser fra humant associerede prøver med vakuumassisteret sorbentekstraktion
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Indfangning af aktivt producerede mikrobielle flygtige organiske forbindelser fra humant associerede prøver med vakuumassisteret sorbentekstraktion

Article DOI: 10.3791/62547-v • 09:19 min • June 1st, 2022
June 1st, 2022 •
1, 1, 2, 3, 3, 3, 1
1Department of Molecular Biology and Biochemistry, University of California Irvine, 2Department of Ecology and Evolutionary Biology, University of California Irvine, 3Entech Instruments Inc.

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Denne protokol beskriver ekstraktionen af flygtige organiske forbindelser fra en biologisk prøve med den vakuumassisterede sorbentekstraktionsmetode, gaskromatografi kombineret med massespektrometri ved hjælp af Entech Sample Preparation Rail og dataanalyse. Det beskriver også dyrkning af biologiske prøver og stabil isotopsondering.

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Biokemi udgave 184 Flygtige organiske forbindelser GC-MS vakuumassisteret sorbentekstraktion headspace-ekstraktion kliniske prøver stabil isotopsondering
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