A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 01:06Mitochondrial Isolation from the Mouse Heart
- 03:01Preparation of Mitoplasts with a French Press for Mechanical Rupture of the OMM
- 04:07Electrophysiological Recordings of H+ Leak Through UCP1 and AAC
- 08:20Results: The Analysis of FA-Dependent Proton Current via UCP1 in Brown Fat and via AAC in Heart Mitoplast
- 10:05Conclusion
