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使用膜片钳技术研究线粒体的产热能力
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Biology
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JoVE Journal
Biology
The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria
Please note that all translations are automatically generated.
Click here for the English version.
使用膜片钳技术研究线粒体的产热能力
DOI:
10.3791/62618-v
•
11:05 min
•
May 03, 2021
•
Ambre M. Bertholet
1
Department of Physiology, David Geffen School of Medicine
,
University of California Los Angeles
Chapters
00:04
Introduction
01:06
Mitochondrial Isolation from the Mouse Heart
03:01
Preparation of Mitoplasts with a French Press for Mechanical Rupture of the OMM
04:07
Electrophysiological Recordings of H
+
Leak Through UCP1 and AAC
08:20
Results: The Analysis of FA-Dependent Proton Current via UCP1 in Brown Fat and via AAC in Heart Mitoplast
10:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法文章详细介绍了使用膜片钳技术测量穿过线粒体内膜的H
+
泄漏的主要步骤,这是一种研究线粒体产热能力的新方法。
Tags
Patch-clamp
Mitochondria
Thermogenic Capacity
Proton Leak
Inner Mitochondrial Membrane
Calcium
Adenine Nucleotides
Mouse
Heart
Homogenization
Centrifugation
French Press
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