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Journal / Medicine / In vitro Vurdering av hjerte omprogrammering ved…
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JoVE Journal Medicine
In vitro Vurdering av hjerte omprogrammering ved å måle hjertespesifikk kalsiumfluks med en GCaMP3 Reporter
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In vitro Vurdering av hjerte omprogrammering ved å måle hjertespesifikk kalsiumfluks med en GCaMP3 Reporter

Article DOI: 10.3791/62643-v • 05:04 min • February 22nd, 2022
February 22nd, 2022 •
*1,2, *1, 1
1Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, 2Department of Cardiovascular Medicine, Xiangya Hospital, Central South University
* These authors contributed equally

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Vi beskriver her etableringen og anvendelsen av en Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referert til som αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 nedenfor) musereporterlinje for hjerteprogrammeringsvurdering. Neonatal hjertefibroblaster (NCFer) isolert fra musestammen omdannes til induserte kardiomyocytter (iCMer), noe som muliggjør praktisk og effektiv evaluering av omprogrammeringseffektivitet og funksjonell modning av iCMer via kalsium (Ca2 +) flux.

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