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Isogen nyre glomerulus chip konstrueret fra humaninducerede pluripotente stamceller
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
DOI:

10:23 min

November 04, 2022

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Chapters

  • 00:04Introduction
  • 00:59Preparation of Microfluidic Organ Chip Devices
  • 01:31Seeding of viECs and Intermediate Mesoderm Cells into the Microfluidic Device
  • 07:15Podocyte Induction and Chip Maintenance
  • 08:03Results: Differentiation of viECs and Podocytes on Organ-On-A-Chip Device
  • 09:35Conclusion

Summary

Automatic Translation

Præsenteret her er en protokol til at konstruere et personligt organ-on-a-chip-system, der rekapitulerer strukturen og funktionen af den nyreglomerulære filtreringsbarriere ved at integrere genetisk matchede epitel- og vaskulære endotelceller differentieret fra humaninducerede pluripotente stamceller. Dette bioengineered system kan fremme nyrepræcisionsmedicin og relaterede applikationer.

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