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Journal
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Immunology and Infection
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间充质干细胞调节巨噬细胞吞噬作用;定量和成像
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JoVE Journal
Immunology and Infection
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
Please note that all translations are automatically generated.
Click here for the English version.
间充质干细胞调节巨噬细胞吞噬作用;定量和成像
DOI:
10.3791/62729-v
•
09:10 min
•
July 16, 2021
•
Jodi F. Evans
,
Anthony E. Ricigliano
,
Anthony V. Morante
,
Emily Martinez
,
Darlisy Vargas
,
Jonah Thyagaraj
1
Molloy College
Chapters
00:04
Introduction
00:50
Mesenchymal Stem Cells Culture in Experimental Dish
03:01
Activation of MΦ (Macrophage Cells) with IFN-γ
03:44
MΦ (Macrophage Cells) Isolation and Co-culture with Mesenchymal Stem Cells
04:53
Zymosan Preparation and Addition to the Co-cultured Cells
06:09
Phagocytosis Assay and Dynamic Imaging
07:57
Results: Effect of IFN-γ on Phagocytosis
08:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里提出的方案用于量化和生成间充质干细胞(MSC)介导的巨噬细胞(MΦ)吞噬作用的动态图像,这些聚氧菌(zymosan)颗粒与pH敏感荧光分子偶联。
Tags
Mesenchymal Stem Cells
Macrophage Phagocytosis
Cell Culture
Co-culture
Bioparticles
PH-sensitive Dyes
Phagocytes
Cell Counting
Trypan Blue
Hemocytometer
Cell Suspension
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