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JoVE Journal Neuroscience
Fluorescerende måling i realtid af synaptiske funktioner i modeller af amyotrofisk lateral sklerose
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Fluorescerende måling i realtid af synaptiske funktioner i modeller af amyotrofisk lateral sklerose

Article DOI: 10.3791/62813-v • 08:59 min • July 16th, 2021
July 16th, 2021 •
*1, 1, 1, *1
1Jefferson Weinberg ALS Center, Thomas Jefferson University
* These authors contributed equally

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To relaterede metoder er beskrevet for at visualisere subcellulære hændelser, der kræves til synaptisk transmission. Disse protokoller muliggør realtidsovervågning af dynamikken i præsynaptisk calciumtilstrømning og synaptisk vesikelmembranfusion ved hjælp af levende cellebilleddannelse af in vitro-dyrkede neuroner.

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Neurovidenskab udgave 173
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