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Retrograd fluorescensmærkning Tillader Målrettet Extracellular Single-unit Optagelse fra Identificerede Neuroner<em> In vivo</em
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograd fluorescensmærkning Tillader Målrettet Extracellular Single-unit Optagelse fra Identificerede Neuroner<em> In vivo</em

DOI:
10.3791/3921-v

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12:32 min

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June 26, 2013

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, , ,
1Department of Biology,Washington University in St. Louis , 2Division of Biological Science, Graduate School of Science,Nagoya University, 3Department of Psychiatry,Washington University in St. Louis

Chapters

  • 00:05Title
  • 02:26Pre-surgical Preparation
  • 04:08Surgery
  • 06:10Retrograde Labeling and Visualization of the Axon of Interest
  • 08:27Extracellular Activity Recording
  • 10:58Representative Results
  • 11:43Conclusion

Summary

Automatic Translation

Automatic Translation

Retrograd transport af fluorescerende farvestof mærker en sub-population af neuroner baseret på anatomiske projektion. Mærkede axoner kan visuelt målrettes<em> In vivo</em>, Tillader ekstracellulær optagelse fra identificerede axoner. Denne teknik gør det lettere at optage, når neuroner ikke kan mærkes gennem genetisk manipulation eller er vanskelige at isolere med 'blinde'<em> In vivo</em> Tilgange.

Tags

Retrograde Fluorescent LabelingTargeted Extracellular Single-unit RecordingIdentified NeuronsIn Vivo Electrophysiological RecordingsNeural CircuitsCellular IdentityIntracellular DyeJuxtacellular IontophoresisLoose-patch IontophoresisFluorescent ProteinsVisually-guided Single-cell ElectrophysiologyGenetic ToolsPromoterProjection PatternsViral VectorsTrans-synaptic SpecificityPre-visualizationTarget Neuron

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