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Isolasjon og kultur av primære humane gingival epitelceller ved hjelp av Y-27632
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1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine,Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology,Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology,Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery,Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School,Hangzhou Medical College
Chapters
- 00:00Introduction
- 00:49Gingival Tissue Pretreatment
- 01:13Digestion of Gingival Tissue
- 02:46Cell Passage
- 03:51Representative Results
- 06:18Conclusion
Her presenterer vi en modifisert metode for isolasjon og kultur av menneskelige gingival epitelceller ved å legge rockhemmeren, Y-27632, til den tradisjonelle metoden. Denne metoden er enklere, mindre tidkrevende, forbedrer stamcelleegenskapene, og produserer et større antall høypotensiale epitelceller både for laboratoriet og for kliniske anvendelser.
