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焦磷酸测序:一个准确的基因分型的简单方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Pyrosequencing: A Simple Method for Accurate Genotyping
DOI:

13:06 min

January 08, 2008

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Chapters

  • 00:00Title
  • 00:40Introduction
  • 02:19Preparing the PCR Product for Pyrosequencing
  • 06:42Pyrosequencing
  • 06:45Entering Assay Details Using Pyrosequencing 96A1.1 Software
  • 08:36Run Sample Plate
  • 11:16Examining Pyrosequencing Results
  • 12:18Conclusion

Summary

Automatic Translation

焦磷酸测序(R)是其中一个最彻底而又简单的方法用于分析基因多态性。此方法具有快速,高效的单核苷酸多态性的评价,包括很多临床相关基因多态性。焦磷酸测序的技术和方法进行了解释。

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