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Journal / Biochemistry / Elaborazione dei campioni di Shotgun Proteomics…
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Biochemistry

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JoVE Journal Biochemistry
Elaborazione dei campioni di Shotgun Proteomics automatizzata da un robot da laboratorio open source
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Elaborazione dei campioni di Shotgun Proteomics automatizzata da un robot da laboratorio open source

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Protocollo dettagliato e tre script Python sono forniti per il funzionamento di un sistema di gestione dei liquidi robotico open source per eseguire la preparazione semi-automatizzata di campioni proteici per esperimenti di spettrometria di massa, che coprono la rimozione del detergente, la digestione delle proteine e le fasi di desalinizzazione dei peptidi.

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Biochimica Numero 176
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