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Bioengineering

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JoVE Journal Bioengineering
Análise espacial bidimensional automatizada de sondas FRET de molécula única móvel
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Análise espacial bidimensional automatizada de sondas FRET de molécula única móvel

Article DOI: 10.3791/63124-v • 08:26 min • November 23rd, 2021
November 23rd, 2021 •
1, 2, 2, 2, 1
1Institute of Applied Physics, TU Wien, 2Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna

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Este artigo apresenta um método para análise espiostemporal de sondas móveis, de uma molécula, baseadas em Förster, utilizando microscopia de fluorescência widefield. O kit de ferramentas de software recém-desenvolvido permite a determinação de traços de tempo smFRET de sondas móveis, incluindo a eficiência correta do FRET e as posições moleculares, em função do tempo.

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Bioengenharia Edição 177
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