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Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
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1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas,Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Vitreo-retinal Department,Centro Privado de Ojos Romagosa-Fundación VER, 4Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Chapters
- 00:13Introduction
- 01:19Fluorescent Lectin Statining
- 03:31Confocal Microscopy Visualization and Microphotograph Acquisition
- 04:14Image Processing
- 05:31Quantification of Avascular Areas
- 06:01Quantification of Neovascular Areas
- 06:27Quantification of Vessel Diameter
- 06:57Quantification of Vessel Tortuosity
- 07:44Quantification of Vascular Branching
- 08:16Quantification of Vascular Density
- 08:43Results
- 12:00Conclusion
This article describes a well-established and reproducible lectin stain assay for the whole mount retinal preparations and the protocols required for the quantitative measurement of vascular parameters frequently altered in proliferative and non-proliferative retinopathies.
Tags
Vascular ParametersWhole Mount RetinasNon-proliferative RetinopathyProliferative RetinopathyOxygen-induced RetinopathyMetabolic SyndromeVascular AlterationsAvascular AreasNeovascular AreasArteriole DilatationArteriole TortuosityVascular DensityVascular BranchingRetinal DissectionImmunohistochemistryIsolectin Staining
