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用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究
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Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
Please note that all translations are automatically generated.
Click here for the English version.
用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究
DOI:
10.3791/63195-v
•
06:56 min
•
October 08, 2021
•
Meiqi Ma
,
Pei Liu
,
Jingya Yu
,
Runhua Han
,
Letian Xu
1
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences
,
Hubei University
,
2
Institute of Plant Protection
,
Wuhan Institute of Landscape Architecture
,
3
McKetta Department of Chemical Engineering
,
University of Texas at Austin
Chapters
00:05
Introduction
00:54
Insect Rearing
01:43
Germ-Free Poplar Culturing
02:40
Egg Surface Sterilization and Rearing Axenic Larvae
03:47
Verification of Axenic Larvae with Culture-Dependent Assays
04:40
Verification of Axenic Individuals with Culture-Independent Assays
05:32
Results: Confirmation of Efficacy of Gut Bacteria Elimination
06:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
为了获得轴性昆虫,其卵表面被灭菌,然后使用轴性叶子饲养孵化的幼虫。这种方法为抗焦虑昆虫的制备提供了一种有效的方法,而无需施用抗生素或开发人造饮食,这也可以应用于其他食叶昆虫。
Tags
Axenic Insects
Gut Microbiota
Leaf Beetle
Plant Seedlings
Alpha-naphthalene Acetic Acid
MS Medium
Surface Sterilization
Germ-free
Artificial Diet
Insect Rearing
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