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1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies,University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies,University of Edinburgh, 3Centre for Integrative Physiology,University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh
Chapters
- 00:05Title
- 01:32Sample Preparation and Loading
- 03:43iBlot2 Semi Dry Fast Protein Transfer
- 04:54Post Transfer Membrane Handling
- 06:32Visualization
- 07:51Quantification of Scanned Proteins
- 08:50Results: Representative Quantitative Fluorescent Western Blotting Experiments
- 10:31Conclusion
The advancement of western blotting using fluorescence has allowed detection of subtle changes in protein expression enabling quantitative analyses. Here we describe a robust methodology for detection of a range of proteins across a variety of species and tissue types. A strategy to overcome common technical problems is also provided.
