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使用共聚焦显微镜以高时间分辨率记录小鼠神经肌肉接头中的钙瞬变
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
DOI:

11:12 min

December 01, 2021

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Chapters

  • 00:05Introduction
  • 01:12Dye Loading Procedure
  • 03:28Video Capture with Confocal Microscopy
  • 06:14Video Processing
  • 07:56Video Analysis
  • 09:55Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
  • 10:18Conclusion

Summary

Automatic Translation

该方案描述了通过切割神经将荧光钙染料加载到小鼠运动神经末梢的方法。此外,还提出了一种使用共聚焦显微镜记录周围神经末梢快速钙瞬变的独特方法。

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