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Neuroscience
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使用共聚焦显微镜以高时间分辨率记录小鼠神经肌肉接头中的钙瞬变
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用共聚焦显微镜以高时间分辨率记录小鼠神经肌肉接头中的钙瞬变
DOI:
10.3791/63308-v
•
11:12 min
•
December 01, 2021
•
Nikita V. Zhilyakov
,
Arsenii Y. Arkhipov
,
Eduard F. Khaziev
2
,
Marat A. Mukhamedyarov
,
Dmitry V. Samigullin
2
1
Laboratory of Biophysics of Synaptic Processes
,
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS
,
2
Department of Radiophotonics and Microwave Technologies
,
Kazan National Research Technical University named after A.N. Tupolev
,
3
Department of Normal Physiology
,
Kazan State Medical University
Chapters
00:05
Introduction
01:12
Dye Loading Procedure
03:28
Video Capture with Confocal Microscopy
06:14
Video Processing
07:56
Video Analysis
09:55
Results: Mouse Nerve Terminals Loaded with the Ca
2+
Indicator and Representative Trace of Calcium Signal
10:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了通过切割神经将荧光钙染料加载到小鼠运动神经末梢的方法。此外,还提出了一种使用共聚焦显微镜记录周围神经末梢快速钙瞬变的独特方法。
Tags
Calcium Transients
Neuromuscular Junction
Confocal Microscopy
Presynaptic Calcium
Synaptic Transmission
Calcium Signaling
Neurodegenerative Diseases
Loading Technique
Spatial-temporal Resolution
Confocal Microscope
Nerve Trunk
Nerve Stump
Dye Loading Solution
Article
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