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JoVE Journal Neuroscience
Registrazione di transitori di calcio nella giunzione neuromuscolare di topo ad alta risoluzione temporale mediante microscopia confocale
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Registrazione di transitori di calcio nella giunzione neuromuscolare di topo ad alta risoluzione temporale mediante microscopia confocale

Article DOI: 10.3791/63308-v • 11:12 min • December 1st, 2021
December 1st, 2021 •
1, 1, 1,2, 3, 1,2
1Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology, Kazan State Medical University

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Il protocollo descrive il metodo di caricamento di un colorante di calcio fluorescente attraverso il nervo tagliato nei terminali nervosi motori del topo. Inoltre, viene presentato un metodo unico per registrare i transitori di calcio veloci nelle terminazioni nervose periferiche utilizzando la microscopia confocale.

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Neuroscienze Numero 178
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