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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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JoVE Journal Biology
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

DOI:
10.3791/63405-v

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05:48 min

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March 16, 2022

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, , ,
1Nora Eccles Harrison Cardiovascular Research and Training Institute,University of Utah, 2Department of Biomedical Sciences,Cedars-Sinai Medical Center

Chapters

  • 00:04Introduction
  • 00:42DNA Extraction
  • 01:45DNA Amplification by PCR
  • 02:42Incubation with Restriction Enzyme
  • 03:34Preparation of 1.5% Agarose Gel for Electrophoresis and DNA Band Detection
  • 04:22Results: Genotype Analysis and Recombination Efficiency
  • 05:21Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a single M213L mutation in Gja1 that retains full-length Connexin43 generation but prevents translation of the smaller GJA1-20k internally translated isoform.

Tags

Keywords: MRNAProtein ExpressionGenotypingPCRRestriction EnzymeTail SampleThermal CyclerPCR Master MixNlaIIIAgarose Gel Electrophoresis

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