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Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification
DOI:

07:34 min

February 10, 2022

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Chapters

  • 00:04Introduction
  • 00:41Microdissection of Tricuspid Valve Leaflet Composite Layers
  • 06:04Results: Histological Assessment and Biaxial Mechanical Testing
  • 07:07Conclusion

Summary

Automatic Translation

This protocol describes the biaxial mechanical characterization, polarized spatial frequency domain imaging-based collagen quantification, and microdissection of tricuspid valve leaflets. The provided method elucidates how the leaflet layers contribute to the holistic leaflet behaviors.

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