Journal
/
Biology
/
Zebrafish Corneal Wound Healing: Fra slitasje til sårlukking imaging analyse
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Zebrafish Corneal Wound Healing: From Abrasion to Wound Closure Imaging Analysis

Zebrafish Corneal Wound Healing: Fra slitasje til sårlukking imaging analyse

DOI:
10.3791/63605-v

•

08:19 min

•

March 01, 2022

•

, ,
1Institute of Biotechnology, HiLIFE,University of Helsinki, 2Institute for Neurosciences of Montpellier,Univ Montpellier

Chapters

  • 00:04Introduction
  • 01:01Preparation and Sample Collection
  • 03:03Sample Processing for Electron Microscopy
  • 04:25Imaging and Measuring Cell Shape, Size, and Microridge Pattern
  • 06:36Results: Imaging Analysis Using Scanning Electron Microscopy
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen fokuserer på å skade den okulære overflaten av sebrafisk gjennom slitasje for å vurdere den påfølgende sårlukkingen på cellenivå. Denne tilnærmingen utnytter en okulær burr for delvis å fjerne hornhinnen epitel og bruker skanning elektronmikroskopi for å spore endringer i cellemorfologi under sårlukking.

Tags

ZebrafishCorneal Wound HealingCorneal AbrasionWound ClosureCorneal TransparencyPathogenesisOphthalmic BurrTricaineAnesthesiaAnalgesicGlutaraldehydeSodium Phosphate Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis

Development of automated imaging and analysis for zebrafish chemical screens.

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish

Metabolsk profil Analyse av sebrafisk embryo

Analyse av Embryonale og Larvesebrafisk Skeletal myofibers fra Dissosierte Forberedelser

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

Metoder for Skin såre og Analyser for Wound Responses i<em> C. elegans</em

Nyreskade Mikroinjeksjon i Sebrafisk til Model Akutt nyreskade

Analyse av Beta-celle-funksjonen bruker encellede oppløsning kalsium Imaging i sebrafisk holmer

Read Article