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白色念珠菌
中转录因子-DNA结合相互作用的全基因组分析:全面的CUT&RUN方法和数据分析工作流程
JoVE Journal
Biology
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JoVE Journal
Biology
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in
Candida albicans
: A Comprehensive CUT&RUN Method and Data Analysis Workflow
Please note that all translations are automatically generated.
Click here for the English version.
白色念珠菌
中转录因子-DNA结合相互作用的全基因组分析:全面的CUT&RUN方法和数据分析工作流程
DOI:
10.3791/63655-v
•
07:48 min
•
April 01, 2022
•
Mohammad N. Qasim*
1,2
,
Ashley Valle Arevalo*
1,2
,
Akshay D. Paropkari*
1,2
,
Craig L. Ennis
2,4
,
Suzanne S. Sindi
,
Clarissa J. Nobile
4
,
Aaron D. Hernday
4
1
Department of Molecular and Cell Biology
,
University of California, Merced
,
2
Quantitative and Systems Biology Graduate Program
,
University of California, Merced
,
3
Department of Applied Mathematics
,
University of California, Merced
,
4
Health Sciences Research Institute
,
University of California, Merced
Chapters
00:04
Introduction
00:49
Isolation of Nuclei and Primary Antibody Binding
02:40
Binding of pAG-MNase to Antibody
03:36
Library Preparation for Sequencing
05:22
CUT&RUN Sequence Analysis
05:54
Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
07:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种实验方法和数据分析工作流程,用于在靶标下切割并使用核酸酶(CUT&RUN)在人类真菌病原体
白色念珠菌
中释放。
Tags
Genome-wide Profiling
Transcription Factor-DNA Binding
Candida Albicans
CUT&RUN
ChIP-chip
ChIP-seq
Epitope Tagging
Nuclei Isolation
Fluorescence Microscopy
Antibody Incubation
Protein AG-MNase
Data Analysis Workflow
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