-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

EN

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

English

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Environment
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Full Text
3,283 Views
06:22 min
March 25, 2022

DOI: 10.3791/63737-v

Hiroshi Arai1, Yuna Ishitsubo1, Madoka Nakai1, Maki N. Inoue1

1Department of Applied Biological Science,Tokyo University of Agriculture and Technology

The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.

We present fundamental, but highly in demand masses for mass rearing, observations and molecular studies using serious pest Tortrix masses, to study their biology. So far, the structure of the insect eggs have prevented further analysis. Notably, we enabled the study of thin, soft and fragile eggs covered by maternal secretion.

There is simple techniques expected to be applicable for further research on tortrix, trying other insects and other taxa. Begin by placing a female and two males in a 120 milliliter plastic cup with a piece of paraffin paper to establish a Matra line. Slice approximately 60 grams of artificial diets using a Grater for mass rearing.

View the full transcript and gain access to thousands of scientific videos

View the full transcript and gain access to thousands of scientific videos

Sign In Start Free Trial

Explore More Videos

Mass RearingTortricidaePest InsectsMolecular StudiesEgg MassArtificial DietMating TechniqueEthanol HydrophilizationDAPI SolutionEgg VisualizationLarvae DissectionParaffin PaperGenetic AnalysisEmbryonic Studies

Related Videos

Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil

17:41

Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil

Related Videos

25.6K Views

Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies

08:53

Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies

Related Videos

18.3K Views

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Related Videos

11.3K Views

Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System

09:23

Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System

Related Videos

12.5K Views

Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera (LeConte)

07:38

Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera (LeConte)

Related Videos

6.5K Views

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

06:08

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Related Videos

5K Views

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Related Videos

5.1K Views

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

08:30

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

Related Videos

3.9K Views

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

04:41

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Related Videos

2.3K Views

Isolation, Behavioral Identification, and Pathogenicity Assessment of Entomopathogenic Fungi from a Forest Wood Borer

06:58

Isolation, Behavioral Identification, and Pathogenicity Assessment of Entomopathogenic Fungi from a Forest Wood Borer

Related Videos

1.4K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code