Journal
/
Environment
/
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

DOI:
10.3791/63737-v

•

06:22 min

•

March 25, 2022

•

, , ,
1Department of Applied Biological Science,Tokyo University of Agriculture and Technology

Chapters

  • 00:05Introduction
  • 00:42Insect Collection and Mass Rearing
  • 01:32Separation of Eggs and Pharate Larvae from Egg Masses for Fixation, Permeabilization, Staining, and DNA and RNA Extraction from Pharate Larvae
  • 04:35Results: Morphological Observation of Embryos and Nucleic Acid Extraction
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.

Tags

Keywords: TortricidaeMass RearingMolecular StudiesPest InsectsInsect EggsArtificial DietEgg MassSodium HypochloritePotassium HydroxideHeptaneParaformaldehydeMethanolEthanolDAPILactic AcidAcetic AcidLarval Dissection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63737?article_type=v

Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies
check_url/63737?article_type=v

Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
check_url/63737?article_type=v

Vegetated Treatment Systems for Removing Contaminants Associated with Surface Water Toxicity in Agriculture and Urban Runoff
check_url/63737?article_type=v

Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
check_url/63737?article_type=v

Procedures of Laboratory Fumigation for Pest Control with Nitric Oxide Gas
check_url/63737?article_type=v

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
check_url/63737?article_type=v

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
check_url/63737?article_type=v

Harvesting Venom Toxins from Assassin Bugs and Other Heteropteran Insects
check_url/63737?article_type=v

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
check_url/63737?article_type=v

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Read Article