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DOI: 10.3791/63737-v
The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.
We present fundamental, but highly in demand masses for mass rearing, observations and molecular studies using serious pest Tortrix masses, to study their biology. So far, the structure of the insect eggs have prevented further analysis. Notably, we enabled the study of thin, soft and fragile eggs covered by maternal secretion.
There is simple techniques expected to be applicable for further research on tortrix, trying other insects and other taxa. Begin by placing a female and two males in a 120 milliliter plastic cup with a piece of paraffin paper to establish a Matra line. Slice approximately 60 grams of artificial diets using a Grater for mass rearing.
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