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龟科害虫的大规模饲养与分子研究
JoVE Journal
Environment
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JoVE Journal
Environment
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
Please note that all translations are automatically generated.
Click here for the English version.
龟科害虫的大规模饲养与分子研究
DOI:
10.3791/63737-v
•
06:22 min
•
March 25, 2022
•
Hiroshi Arai
,
Yuna Ishitsubo
,
Madoka Nakai
,
Maki N. Inoue
1
Department of Applied Biological Science
,
Tokyo University of Agriculture and Technology
Chapters
00:05
Introduction
00:42
Insect Collection and Mass Rearing
01:32
Separation of Eggs and Pharate Larvae from Egg Masses for Fixation, Permeabilization, Staining, and DNA and RNA Extraction from Pharate Larvae
04:35
Results: Morphological Observation of Embryos and Nucleic Acid Extraction
05:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本方案描述了实验室中Tortricid pest insects的饲养方法。区分昆虫性别和提取核酸以进行高通量测序的程序是使用两种tortricid害虫建立的。
Tags
Tortricidae
Mass Rearing
Molecular Studies
Pest Insects
Insect Eggs
Artificial Diet
Egg Mass
Sodium Hypochlorite
Potassium Hydroxide
Heptane
Paraformaldehyde
Methanol
Ethanol
DAPI
Lactic Acid
Acetic Acid
Larval Dissection
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