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Masseopdræt og molekylære undersøgelser i Tortricidae skadedyrsinsekter
JoVE Journal
Environment
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JoVE Journal Environment
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
DOI:

06:22 min

March 25, 2022

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Chapters

  • 00:05Introduction
  • 00:42Insect Collection and Mass Rearing
  • 01:32Separation of Eggs and Pharate Larvae from Egg Masses for Fixation, Permeabilization, Staining, and DNA and RNA Extraction from Pharate Larvae
  • 04:35Results: Morphological Observation of Embryos and Nucleic Acid Extraction
  • 05:52Conclusion

Summary

Automatic Translation

Den foreliggende protokol beskriver opdrætsmetoden for tortricid skadedyrsinsekter i laboratorierne. Procedurerne til skelnen mellem insekters køn og ekstraktion af nukleinsyrer til sekventering med høj gennemstrømning etableres ved anvendelse af to tortricid skadedyr.

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