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果蝇卵生过程中极化细胞内运输和基底膜蛋白分泌的共聚焦和超分辨率成像
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Developmental Biology
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JoVE Journal Developmental Biology
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
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果蝇卵生过程中极化细胞内运输和基底膜蛋白分泌的共聚焦和超分辨率成像

DOI:
10.3791/63778-v

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10:41 min

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May 19, 2022

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,
1Department of Biological Sciences,Northern Illinois University

Chapters

  • 00:05Introduction
  • 00:44Fluorescence Immunostaining
  • 02:28Mounting of Stained Ovaries
  • 04:04Super-Resolution Image Acquisition Using Airyscan Microscopy
  • 07:073D Reconstruction of Optical Z-Section
  • 08:10Results: Characterization of the Intracellular Trafficking and Secretion of Basement Membrane Proteins in Epithelial Cells
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

基底膜对于发育过程中的组织和器官形态发生至关重要。为了更好地理解导致该结构正确放置的机制，所提出的方案描述了使用共聚焦和超分辨率显微镜可视化和表征上皮细胞中基底膜蛋白的细胞内运输和分泌的方法。

Tags

Confocal MicroscopySuper-resolution ImagingPolarized Intracellular TraffickingBasement Membrane ProteinsDrosophila OogenesisFixationImmunostainingFluorescent Secondary AntibodiesAiryscan Microscopy

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