Journal
/
/
Visualisering af arudvikling ved hjælp af SCAD-assay - Et ex-situ hudardannelsesassay
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Visualizing Scar Development Using SCAD Assay – An Ex-situ Skin Scarring Assay
DOI:

07:40 min

April 28, 2022

, , , ,

Chapters

  • 00:05Introduction
  • 01:03Scar like Tissue in A Dish (SCAD) Preparation
  • 02:50SCAD: Tissue Culture & Live Imaging of SCADs
  • 04:23Tissue Harvesting and 2D/3D Immunofluorescence Staining
  • 05:07Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development
  • 06:43Conclusion

Summary

Automatic Translation

Denne protokol beskriver dannelsen af en hud-fascia-eksplantation nævnt "SCar-lignende væv i en skål" eller SCAD. Denne model tillader hidtil uset visualisering af enkeltfibroblaster under ardannelse.

Related Videos

Read Article