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Journal / Developmental Biology / Generación de esferas 3D y cultivos de interfaz…
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Developmental Biology

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JoVE Journal Developmental Biology
Generación de esferas 3D y cultivos de interfaz aire-líquido 2D de células epiteliales alveolares pluripotentes inducidas por humanos derivadas de células madre pluripotentes de tipo 2
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Journal (Developmental Biology)
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Dissection and Culture of Mouse Embryonic Kidney
Journal (Developmental Biology)
Dissection and Culture of Mouse Embryonic Kidney
 
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Generación de esferas 3D y cultivos de interfaz aire-líquido 2D de células epiteliales alveolares pluripotentes inducidas por humanos derivadas de células madre pluripotentes de tipo 2

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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El presente protocolo describe células alveoidales alveoidales pluripotentes derivadas de células madre pluripotentes inducidas por humanos (iAT2). Estas células pueden cultivarse como esferas autorrenovadoras en cultivo 3D o adaptarse al cultivo de interfaz aire-líquido (ALI).

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Biología del Desarrollo Número 182
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