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Journal / Developmental Biology / 生成人诱导的多能干细胞衍生的2型肺泡上皮细胞的3D球体和2D气液界面培养物…
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Developmental Biology

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JoVE Journal Developmental Biology
生成人诱导的多能干细胞衍生的2型肺泡上皮细胞的3D球体和2D气液界面培养物
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生成人诱导的多能干细胞衍生的2型肺泡上皮细胞的3D球体和2D气液界面培养物

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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本方案描述了人诱导的多能干细胞衍生的2型肺泡上皮样细胞（iAT2s）。这些细胞可以在3D培养物中作为自我更新球体培养或适应气液界面（ALI）培养。

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发育生物学，第182期，
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