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Journal / Developmental Biology / İnsan Kaynaklı Pluripotent Kök Hücre Kaynaklı Tip …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
İnsan Kaynaklı Pluripotent Kök Hücre Kaynaklı Tip 2 Alveolar Epitel Hücrelerinin 3D Küreleri ve 2D Hava-Sıvı Arayüz Kültürlerinin Üretilmesi
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İnsan Kaynaklı Pluripotent Kök Hücre Kaynaklı Tip 2 Alveolar Epitel Hücrelerinin 3D Küreleri ve 2D Hava-Sıvı Arayüz Kültürlerinin Üretilmesi

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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Mevcut protokol, insan kaynaklı pluripotent kök hücre kaynaklı tip 2 alveoler epitel benzeri hücreleri (iAT2'ler) tanımlamaktadır. Bu hücreler 3D kültürde kendini yenileyen küreler olarak kültürlenebilir veya hava-sıvı arayüzü (ALI) kültürüne uyarlanabilir.

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