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长期培养用人心肌组织的制备
JoVE Journal
Medicine
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JoVE Journal Medicine
Preparation of Human Myocardial Tissue for Long-Term Cultivation
DOI:

10:58 min

June 02, 2022

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Chapters

  • 00:04Introduction
  • 01:01Preparing Agarose and Vibratome
  • 02:01Trimming and Embedding the Samples
  • 03:39Placing the Samples on Cutting Tray and Setting Vibratome
  • 04:17Medium and Incubator Preparation During Slicing Procedure
  • 05:07Preparing and Mounting Slices
  • 07:06Changing Medium
  • 07:51Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
  • 10:15Conclusion

Summary

Automatic Translation

我们提出了一种人心室心肌组织 离体 培养的方案。它允许对收缩力和动力学进行详细分析,以及应用前负荷和后负荷来更密切地模拟 体内 生理环境。

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