JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
长期培养用人心肌组织的制备
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Preparation of Human Myocardial Tissue for Long-Term Cultivation
Please note that all translations are automatically generated.
Click here for the English version.
长期培养用人心肌组织的制备
DOI:
10.3791/63964-v
•
10:58 min
•
June 02, 2022
•
Jules Hamers
2
,
Payel Sen
2
,
Daphne Merkus
2,3
,
Thomas Seidel
5
,
Kun Lu
6
,
Andreas Dendorfer
2
1
Walter-Brendel-Centre of Experimental Medicine
,
University Hospital, LMU Munich
,
2
German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)
,
3
Division of Experimental Cardiology, Department of Cardiology, Erasmus MC
,
University Medical Center Rotterdam
,
4
Institute of Cellular and Molecular Physiology
,
Friedrich-Alexander-Universität Erlangen-Nürnberg
,
5
Muscle Research Center Erlangen (MURCE)
,
Friedrich-Alexander-Universität Erlangen-Nürnberg
,
6
Department of Cardiac Surgery
,
University Hospital, LMU Munich
Chapters
00:04
Introduction
01:01
Preparing Agarose and Vibratome
02:01
Trimming and Embedding the Samples
03:39
Placing the Samples on Cutting Tray and Setting Vibratome
04:17
Medium and Incubator Preparation During Slicing Procedure
05:07
Preparing and Mounting Slices
07:06
Changing Medium
07:51
Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
10:15
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提出了一种人心室心肌组织
离体
培养的方案。它允许对收缩力和动力学进行详细分析,以及应用前负荷和后负荷来更密切地模拟
体内
生理环境。
Tags
Long-term Cultivation
Biomimetic Chamber
Stimulation Protocols
Cardiovascular Medicine
Slicing Buffer
Agarose Embedding
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
供体角膜内皮角膜移植术的组织准备
封装产热前脂肪细胞移植到脂肪组织仓库
与人体组织转化为癌症研究工作
在新生儿猪淋巴的肠淋巴躯干和收集的长期导尿
导管手术放置了长期的心血管运动试验猪
自由活动小鼠使用遥测长期血压测量
3D全心脏心肌组织分析
组织和采血程序标准化改善闭合性胸猪心肌梗死模型的研究
分析长期心电图记录以检测小鼠心律失常
Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
Read Article