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1Walter-Brendel-Centre of Experimental Medicine,University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC,University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology,Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery,University Hospital, LMU Munich
Chapters
- 00:04Introduction
- 01:01Preparing Agarose and Vibratome
- 02:01Trimming and Embedding the Samples
- 03:39Placing the Samples on Cutting Tray and Setting Vibratome
- 04:17Medium and Incubator Preparation During Slicing Procedure
- 05:07Preparing and Mounting Slices
- 07:06Changing Medium
- 07:51Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
- 10:15Conclusion
Presentiamo un protocollo per la coltivazione ex vivo del tessuto miocardico ventricolare umano. Consente un'analisi dettagliata della forza di contrazione e della cinetica, nonché l'applicazione di pre- e postcarico per imitare più da vicino l'ambiente fisiologico in vivo .
