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从血浆和实体组织中分离和分析可追溯和功能化的细胞外囊泡
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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
Please note that all translations are automatically generated.
Click here for the English version.
从血浆和实体组织中分离和分析可追溯和功能化的细胞外囊泡
DOI:
10.3791/63990-v
•
09:57 min
•
October 17, 2022
•
Yuan Cao*
1,2
,
Ji-Yu Qiu*
1,3
,
Da Chen
2
,
Chen-Yao Li
4
,
Shu-Juan Xing
5
,
Chen-Xi Zheng
,
Xin Liu
,
Yan Jin
6
,
Bing-Dong Sui
1
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology
,
The Fourth Military Medical University
,
2
Department of Orthodontics, School of Stomatology
,
The Fourth Military Medical University
,
3
Department of VIP Dental Care, School of Stomatology
,
The Fourth Military Medical University
,
4
School of Basic Medicine
,
The Fourth Military Medical University
,
5
College of Life Science
,
Northwest University
,
6
Xi’an Institute of Tissue Engineering and Regenerative Medicine
Chapters
00:05
Introduction
01:20
Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
02:50
Origin Analysis of Extracellular Vesicles
05:17
Protein Content Analysis of Extracellular Vesicles
08:06
Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
09:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了一种从外周血和实体组织中提取细胞外囊泡的方法,随后对表面抗原和蛋白质货物进行分析。
Tags
Extracellular Vesicles
Isolation
Analysis
Plasma
Surface Antigens
Protein Cargoes
Flow Cytometry
Maxillary Bone
Liberase
Centrifugation
PBS
Sample Processing
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