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Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices
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JoVE Journal Neuroscience
Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

DOI:
10.3791/64013-v

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04:08 min

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July 22, 2022

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, , , ,
1Department of Integrative Biology,Oklahoma State University, 2Department of Physiology and Biophysics,University of Colorado Anschutz, 3Advanced Light Microscopy Core,University of Colorado Anschutz

Chapters

  • 00:04Introduction
  • 01:19Staining and Imaging of the Sections
  • 02:55Results: Analyzing Brain Tissues Using CARS Imaging
  • 03:34Conclusion

Summary

Automatic Translation

Automatic Translation

Visualizing myelination is an important goal for many researchers studying the nervous system. CARS is a technique that is compatible with immunofluorescence that can natively image lipids within tissue such as the brain illuminating specialized structures such as myelin.

Tags

Keywords: CARSCoherent Anti-Stokes Raman SpectroscopyMyelinationMyelinLipidsCH2 BondsMicroscopyFluorescence ImagingImmunohistochemistryMultiple SclerosisAgingAutismLaser AlignmentForward CARSEpi CARSConfocal Imaging

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