01:06Antibody Panel Preparation and Antibody Staining
02:35Image Acquisition
04:31Results: Isotopic Purity and Channel Cross-Talk, Staining Images in Different Tissues, and Images of the Staining Before and After Image Preparation
In the era of cancer immunotherapy, interest in elucidating tumor microenvironment dynamics has increased strikingly. This protocol details a mass spectrometry imaging technique with respect to its staining and imaging steps, which allow for highly multiplexed spatial analysis.