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체외 거대한 Unilamellar 소포 내부의 Actin Cytoskeleton 재구성
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
DOI:

10:19 min

August 25, 2022

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Buffers and Proteins Solutions
  • 03:07Preparation of Liposomes Based on the Inverted Emulsion Techniques
  • 05:51Microscopy Observation
  • 08:11Results: Microscopic Images of the Monolayer Lipid Droplet and Encapsulation of Actin Networks Inside Liposomes
  • 09:27Conclusion

Summary

Automatic Translation

이 원고에서는 F-actin 세포 골격을 거대한 unilamellar 지질 소포 (리포솜이라고도 함)로 캡슐화하는 실험 기술과 리포솜 막의 내부 전단지에 피질 생모방 F- 액틴 층을 형성하는 방법을 보여줍니다.

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