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Storstilet gravitaxis-analyse af Caenorhabditis Dauer-larver
JoVE Journal
Behavior
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JoVE Journal Behavior
Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae
DOI:

07:53 min

May 31, 2022

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Chapters

  • 00:05Introduction
  • 00:47Chamber Preparation
  • 01:37Filling the Chambers with Agar
  • 02:33Isolating Dauer Larvae
  • 04:01Adding Dauers to the Chamber
  • 05:03Running and Scoring the Assay
  • 06:38Results: Comparison of Gravitaxis Behavior Across Species
  • 07:14Conclusion

Summary

Automatic Translation

Den nuværende protokol skitserer metoder til udførelse af et stort gravitaxisassay med Caenorhabditis dauer larver. Denne protokol giver mulighed for bedre påvisning af gravitakseadfærd sammenlignet med et pladebaseret assay.

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