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Modelo Experimental de Imunoterapia para Melanoma utilizando Vacinação Tumoral com Citocina Hematopoiética
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Modelo Experimental de Imunoterapia para Melanoma utilizando Vacinação Tumoral com Citocina Hematopoiética

Article DOI: 10.3791/64082-v • 09:15 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1
1La Jolla Institute for Immunology

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O protocolo apresenta um modelo de imunoterapia contra o câncer utilizando a vacinação tumoral baseada em células com melanoma B16-F10 expressando Flt3L. Este protocolo demonstra os procedimentos, incluindo a preparação de células tumorais cultivadas, implantação tumoral, irradiação celular, medição do crescimento tumoral, isolamento de células imunes intratumorais e análise de citometria de fluxo.

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Cancer Research Edição 192 Flt3L DC B16-F10 melanoma vacina tumoral imunidade inata células imunes intratumorais
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