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June 23, 2022
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Summary
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Dit protocol laat zien hoe de kleine extracellulaire blaasjes (hUC-MSC-sEV's) van menselijke navelstreng-afgeleide mesenchymale stamcellen kunnen worden geïsoleerd met een eenvoudige benchtop-instelling op laboratoriumschaal. De grootteverdeling, eiwitconcentratie, sEV-markers en morfologie van geïsoleerde hUC-MSC-sEV's worden gekenmerkt door respectievelijk nanodeeltjestrackinganalyse, BCA-eiwittest, western blot en transmissie-elektronenmicroscoop.
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Cite this Article
Koh, B., Tan, K. L., Chan, H. H., Daniel Looi, Q. H., Lim, M. N., How, C. W., Law, J. X., Foo, J. B. A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells. J. Vis. Exp. (184), e64106, doi:10.3791/64106 (2022).
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